Application of Peristaltic Pump in Chromatography Experiment

2023-04-21 14:58:01

Chromatograph is a kind of mixture separation technology, which is widely used in analytical chemistry, organic chemistry, biochemistry and other fields. Chromatograph is used to separate and determine the multi-component mixture based on the differences in physical and chemical properties of components in the mixture. It can be divided into adsorption chromatography, partition chromatography, ion exchange chromatography and gel filtration chromatography according to chromatographic mechanism.

Specific applications of chromatography include:

Desalination, such as the removal, separation and purification of low molecular weight impurities in high molecular weight solution (such as protein, nucleic acid, polysaccharide, etc.) by gel chromatography, the separation of enzyme, protein, polysaccharide, hormone, alkaloid and other substances, the concentration and determination of purified dilute solution, the molecular weight of high molecular weight and the study of various chromatographic methods and packing performance, etc.

Under natural conditions, limited by the penetration rate of the liquid in the chromatographic column, appropriate external pressure is required to enhance the permeability of the liquid and ensure that the liquid is free from cross contamination.

Therefore, the peristaltic pump is the ideal choice for conveying fluid in chromatographic process, which is often used to control the flow rate of mobile phase, the flow rate of eluent, pumping buffer, extracting sample to be tested, etc. The recommended product BT-100B.